Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.047568 |
Chromosome: | chromosome 1 |
Location: | 2848664 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g017650 | FAP34,RIB30 | Ribbon binding 30 kDa microtuble inner protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGACAGGGCACTTGGCTTGGGCCAGCAAAGCTAAGCCTTAGGCTAAG |
Internal bar code: | CCTGCGGGCGGCACCGAAGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2529 |
LEAP-Seq percent confirming: | 94.5946 |
LEAP-Seq n confirming: | 70 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGAATCGCTGGCTTTCTCA |
Suggested primer 2: | CCTTGGCTTTCTTGTGTGCC |