Insertion junction: CLIP2.047571_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.047571
Chromosome:chromosome 16
Location:6123124
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677250 TEH5 (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase; Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTTCCTCTTTCATCTCCCGCCACCCTGCCCGATGCGGTCTTACCCGC
Internal bar code:GCAACATACAACTCTGTGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:630
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACACGAGGTTGACACTC
Suggested primer 2:GGGTTCCACTACTGTCCACG