| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047603 |
| Chromosome: | chromosome 2 |
| Location: | 6173582 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120001 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGAACGCCACCGTACGGCCCGTACACAGGCGAAGGCGGGTACGAGCC |
| Internal bar code: | GTTCTTTCATGGATGTGAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCATCTTCTCCCTCGCC |
| Suggested primer 2: | CAGCTCATAACGTTCGCAGC |