| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047606 |
| Chromosome: | chromosome 17 |
| Location: | 1818242 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709300 | (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTAAGCGCACGCTGGCAGGGCCTGGGGAGGCTGTCGCCCTCATCCTG |
| Internal bar code: | GGTAGAGGCCTGTTTGGGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1483 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTCTCGAAGCTGGTTGG |
| Suggested primer 2: | CACCACGTGTGCTCAGGTAT |