Insertion junction: CLIP2.047614_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047614
Chromosome:chromosome 8
Location:3785611
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381050 CEP8 Cysteine endopeptidase; (1 of 3) 3.4.22.67 - Zingipain / Zingibain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCGAACCCTGTCCTCCAACTGTCCTCCCCTGCCTCTCCCGCCCGCAGG
Internal bar code:GTGTCTGTTGGAGAGGACGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:629
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCCTACGCAACTACCTA
Suggested primer 2:ACAGCATCACGGCTTCTGAA