| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047614 |
| Chromosome: | chromosome 8 |
| Location: | 3785615 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381050 | CEP8 | Cysteine endopeptidase; (1 of 3) 3.4.22.67 - Zingipain / Zingibain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTTGTAGTAGAACGGAGAATAGCGCAGGCGTCTCGCTTGCCAGCAC |
| Internal bar code: | GGGACTAAGGGGGGGTAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2830 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCATCACGGCTTCTGAA |
| Suggested primer 2: | AGGGCCTACGCAACTACCTA |