Insertion junction: CLIP2.047621_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047621
Chromosome:chromosome 7
Location:5385902
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAGGCCACCATCCAGCAGATGAACCCCGAGGAGCTGTACGAGATGAT
Internal bar code:TTTCTCTACTAGGGACTGCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2544
LEAP-Seq percent confirming:65.4545
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:110

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAATAGAGCTGTCGACG
Suggested primer 2:GCTGCACGCTTCGTATCTTG