| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047626 |
| Chromosome: | chromosome 13 |
| Location: | 2416860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579400 | ERM8 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGTCGTGGCTGCCGCTGCTACTGCCGCCAAAGCCGGCGTCGAAAGC |
| Internal bar code: | AGTCTGCTGGAGTGAGTCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1444 |
| LEAP-Seq percent confirming: | 68.3333 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGCAGGGACTCACAGAG |
| Suggested primer 2: | GCCACAACACTTCCACGTTC |