| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.047630 |
| Chromosome: | chromosome 3 |
| Location: | 391177 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144767 | (1 of 1) IPR002761//IPR014729 - Diphthamide synthase domain // Rossmann-like alpha/beta/alpha sandwich fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCGGCATGAAGCTGCTGCACACGTCCAGCATGTCACCGGTGGCCAG |
| Internal bar code: | TAGCATTTCAGTTATAATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1576 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAGCACACCTTCCCAAT |
| Suggested primer 2: | TTGCCTGATCAGATTGCCGT |