Insertion junction: CLIP2.047635_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047635
Chromosome:chromosome 4
Location:1122290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g215950 CYN57 Cyclophilin 57; (1 of 1) K12737 - peptidyl-prolyl cis-trans isomerase SDCCAG10 [EC:5.2.1.8] (SDCCAG10) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAAAACCAACTGGGGCCATAATCCGACAAAGTCGACGCGCATTCGCCC
Internal bar code:GTAATAGCGTAATTTTGTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:805
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGCGTGACGTCATAGCAT
Suggested primer 2:ACCTGTGCCACAATCGTGAT