Insertion junction: CLIP2.047637_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047637
Chromosome:chromosome 13
Location:1349007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571450 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGCATATACCAACGCTGAGGTACGGGGTACTTGATCGGTTGCTGGGT
Internal bar code:GGGCTTGAACTTGGGATATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4566
LEAP-Seq percent confirming:92.9412
LEAP-Seq n confirming:158
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:170

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCTACAATTTTCCCAGG
Suggested primer 2:TACGGCGGTGAACAGTTACC