Insertion junction: CLIP2.047641_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047641
Chromosome:chromosome 11
Location:2473882
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095113 DIV24,TFCE1,TFC-E Tubulin-folding cofactor subunit; (1 of 1) IPR000938//IPR029071 - CAP Gly-rich domain // Ubiquitin-related domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAACATCCGTAAACCGTCGCTTTGCTGCATCGCATACACTGTCTTTGCG
Internal bar code:TGATTCACACATACAGACCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4562
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAACCCCTTCTGTCTCGC
Suggested primer 2:GCTTGCTTTGCGTTCTGGAA