Insertion junction: CLIP2.047644_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047644
Chromosome:chromosome 10
Location:537879
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421250 EXO70 (1 of 1) K07195 - exocyst complex component 7 (EXOC7, EXO70); Component of the Exocyst Complex intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCAACACACACGACCGTGTCCGCGACCCAGCGCGCACACACATTAGTG
Internal bar code:ACCTTGGCTCTCTTGTCAGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:9.52381
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGTACGGTATATGCGCAA
Suggested primer 2:TGCTGATGATCGTACCGTCG