| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047647 |
| Chromosome: | chromosome 3 |
| Location: | 4318973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174400 | CDO1 | Cysteine dioxygenase, regulated by copper nutrition; (1 of 2) 1.13.11.20 - Cysteine dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTAAACCCTACCTGCTGTCACTCACGCAAGCTACTAATCGCACTTGT |
| Internal bar code: | AATGGGGAAGGGGCTTCCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4478 |
| LEAP-Seq percent confirming: | 67.0103 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGAAGGGAAGGTCAGC |
| Suggested primer 2: | AGAAACCCAGTCGCTTCAGG |