| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047656 |
| Chromosome: | chromosome 7 |
| Location: | 1090800 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320200 | (1 of 1) PTHR23244:SF208 - F14M2.9 PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGACGGTTCAGCTGAACTCGACAGACCGAGCTCCTTGCATCTGTCATT |
| Internal bar code: | AGGTAACTTACCAAAGATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAATGCAGGTAGGCTTGG |
| Suggested primer 2: | GAGCATCAGAGGTCAGGACG |