| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047663 |
| Chromosome: | chromosome 4 |
| Location: | 3584625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228550 | (1 of 2) PTHR31140//PTHR31140:SF1 - FAMILY NOT NAMED // B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGCGGAACAAGATCTTCATGGAGGTACGGGCCGGGGGGGGGAGATC |
| Internal bar code: | TGCACGCACGGGAGTGTGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1151 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGGGGAGAGGTGGATCT |
| Suggested primer 2: | GGCCTGTTCTCCAAGCTCAT |