| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047676 |
| Chromosome: | chromosome 16 |
| Location: | 1827473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655400 | (1 of 1) K17985 - activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCCACCAGCCTCAACAGCTTCATTCCCAGCAGCCCGAACCCCTGGC |
| Internal bar code: | CATAAGCCATGTAGTGTATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1187 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTACACCTCCACCAC |
| Suggested primer 2: | CACCGCTGACTGTACCTTGT |