| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047684 |
| Chromosome: | chromosome 16 |
| Location: | 1989522 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656551 | TRL14,TRXL14,PDI | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACGGTGGTTGACATGGCCGCCGAGATGGTGACGGACGCGCATGTACG |
| Internal bar code: | GGCGGGAATATGTGTATCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5358 |
| LEAP-Seq percent confirming: | 95.7447 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCAGTGCCGGTATGAA |
| Suggested primer 2: | CATCCGCAGCTACCATGCTA |