| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047686 |
| Chromosome: | chromosome 6 |
| Location: | 7945817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304250 | RLS2 | RegA/Rls-like protein 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGCTGTGGCTACTGCCAACGCCAGCGCCGCTGCCGTGTGCCTCGCT |
| Internal bar code: | GCGCTTTCCACAGGAATAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3155 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGAGGTTGAAGGAGCG |
| Suggested primer 2: | GTCACCACGACTGTGTAGGG |