Insertion junction: CLIP2.047700_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.047700
Chromosome:chromosome 11
Location:3528158
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477750 (1 of 1) IPR016201 - Plexin-like fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGAGACAAAGAAGCGGGAACAGCTTCCGTTGGCGCTGTAAGGGCCCAG
Internal bar code:CGTGCGCAATCGTTAAAGCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1724
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCCCCTTTCTGGCTCTG
Suggested primer 2:TCTTCGTCATGCTTCCACCC