Insertion junction: CLIP2.047705_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047705
Chromosome:chromosome 13
Location:5053426
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g606450 TNP36,CSB55 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAGCAGTCGTTGCAGACTTTGACCGACCAGGGCCGCTGCCCCTCCTT
Internal bar code:GTTGGCCCGCTCGAATAGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2351
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTAGGTCTTCACGGCC
Suggested primer 2:CCTGCTCCATACCAACACGT