Insertion junction: CLIP2.047705_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047705
Chromosome:chromosome 13
Location:5053429
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g606450 TNP36,CSB55 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGACCTGGGTGAGGACCGTGCATGTGCGGGGACGGCTGGGCACTGGGG
Internal bar code:CCTGGGCGGTTTTTCCGGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2140
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCTCCATACCAACACGT
Suggested primer 2:TTGTGTAGGTCTTCACGGCC