| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047711 |
| Chromosome: | chromosome 12 |
| Location: | 2678120 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505250 | FAP288 | (1 of 3) PF13202//PF13499 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_7); EF Hand Containing Flagellar Associated Protein 288 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGACAAGGACATCACGGTCTCTTCCATGGTGCACAGGTCAAGCGTC |
| Internal bar code: | AACATTGACCCGTCAAGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2425 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACTAGGGTGTGGGATA |
| Suggested primer 2: | CCCAAATCCACATCCTGCCT |