Insertion junction: CLIP2.047741_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047741
Chromosome:chromosome 3
Location:8420190
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g213313 (1 of 2) K00059 - 3-oxoacyl- (fabG) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGTGCAGTTGTCACAGCGTGCGCGCCCGCCCGCCCGCTGCCTCACAC
Internal bar code:CTCTAGGTAGGTAGGGGCCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:51.1628
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCTCACAACTGACGTAT
Suggested primer 2:GTCCATGGACTCACGAGGTC