Insertion junction: CLIP2.047741_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047741
Chromosome:chromosome 3
Location:8420207
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g213313 (1 of 2) K00059 - 3-oxoacyl- (fabG) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTTGCTCCGTCAGACCACATGCGCACGGGACAGGCGCGGCTCACTC
Internal bar code:TGTATGATTGCGCAATGAATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3653
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCATGGACTCACGAGGTC
Suggested primer 2:TGCGCTCACAACTGACGTAT