| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047751 |
| Chromosome: | chromosome 9 |
| Location: | 3010095 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393580 | (1 of 2) PF03097 - BRO1-like domain (BRO1) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCGCACCGGCGCCGTCCAGTAGCACAGGACATATGGATGTGGGGGG |
| Internal bar code: | GAGACCGTGTCATCTGGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1877 |
| LEAP-Seq percent confirming: | 24.812 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTGTCTCAACCACGGTT |
| Suggested primer 2: | AGCACTATTCCCTAGCCCCA |