Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.047771 |
Chromosome: | chromosome 7 |
Location: | 5231817 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g349152 | (1 of 2) IPR000104//IPR011598 - Antifreeze protein, type I // Myc-type, basic helix-loop-helix (bHLH) domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCATAGACGTTCCGCCACAAGAAAGAAACAATACACGTGTGAACCAC |
Internal bar code: | TATGACACCTTTTTTATAGTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4032 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 40 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCACACAACAGCAGCACAG |
Suggested primer 2: | GGTGACTTGAGCTAGCTGCA |