Insertion junction: CLIP2.047771_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.047771
Chromosome:chromosome 7
Location:5231817
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g349152 (1 of 2) IPR000104//IPR011598 - Antifreeze protein, type I // Myc-type, basic helix-loop-helix (bHLH) domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCATAGACGTTCCGCCACAAGAAAGAAACAATACACGTGTGAACCAC
Internal bar code:TATGACACCTTTTTTATAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4032
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACACAACAGCAGCACAG
Suggested primer 2:GGTGACTTGAGCTAGCTGCA