| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.047783 |
| Chromosome: | chromosome 2 |
| Location: | 7831847 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146250 | NFS1,NIFS1,CSD1 | (1 of 1) PTHR11601:SF34 - CYSTEINE DESULFURASE, MITOCHONDRIAL; Cysteine desulfurase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCACCCAATATCCCGGCCCGCAGCTCTGCGCCATAGTTCTCTTCAC |
| Internal bar code: | GGTTCAATCACTGGCATGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCACATGCATCCGCCAAC |
| Suggested primer 2: | CTGTCCAACACGCACTTGTG |