Insertion junction: CLIP2.047789_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.047789
Chromosome:chromosome 7
Location:2752872
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g331300 AGP3 ADP-glucose pyrophosphorylase large subunit; (1 of 1) PF00132//PF00483 - Bacterial transferase hexapeptide (six repeats) (Hexapep) // Nucleotidyl transferase (NTP_transferase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGACGTGGGCAACTCCATTCGCACCTACAAGGAGGTGTGTGGGGGGGA
Internal bar code:TGTTAATGCTAGAGAATTGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCCCTCTTCCCCCTCATC
Suggested primer 2:GGTGTGTGTGTGTGTGTGTG