| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047791 |
| Chromosome: | chromosome 16 |
| Location: | 6580143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674050 | ADH12 | Alcohol dehydrogenase; (1 of 1) 1.6.5.5 - NADPH:quinone reductase / Zeta-crystallin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCTTATGGGTGGGAGCCTGGGAGGGAAGGTCCTCAGTACTGGGGCC |
| Internal bar code: | ACTTGTTTAGTCTCGACACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3222 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACAGCCACAGTCACAA |
| Suggested primer 2: | TTTGGTTTGGGCTGGTGTCT |