| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047815 |
| Chromosome: | chromosome 10 |
| Location: | 945522 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424500 | CPK4 | (1 of 1) K00852 - ribokinase (rbsK, RBKS); putative carbohydrate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGGGACGACCCCAACGCCAGCCTGCTACGCAACGCACTGCTGGGAT |
| Internal bar code: | AATCCGGGTGTCACCATTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 9.67742 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCCACAATGATGATGCTG |
| Suggested primer 2: | GAACGGCGTAGACATGTTGC |