| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047818 |
| Chromosome: | chromosome 8 |
| Location: | 298380 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358569 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCCAATTCCAGCCTGATCCGCCCACACACTATCTACACGATGTAAT |
| Internal bar code: | TGCCGGAAGGAGCCGTGTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1195 |
| LEAP-Seq percent confirming: | 30.5085 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCGCTGCCGTTTACAGA |
| Suggested primer 2: | TGAGCACAAACGAACAAGCG |