| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047824 |
| Chromosome: | chromosome 13 |
| Location: | 2578274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580550 | HLM20 | (1 of 2) K11426 - SET and MYND domain-containing protein (SMYD); putative N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGAACTGAACTGGTGAAACAATTGCGGGAGCAAGCCTGCAACAGTTT |
| Internal bar code: | CGGCTGCTTTTGACCAAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1188 |
| LEAP-Seq percent confirming: | 83.6735 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGAGTCAATGCCGATGT |
| Suggested primer 2: | ACGAGGCAAAACTCCTGGAG |