Insertion junction: CLIP2.047825_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.047825
Chromosome:chromosome 12
Location:2579250
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g506650 (1 of 1) PF07064 - RIC1 (RIC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCGGCACCACCACCACGCCACCATCACCACCACGCCACCATCACCACC
Internal bar code:TTTTCAACCCTTCAGTTGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:533
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCCCAACTTGGCACGTA
Suggested primer 2:GCGTGTATTTGGTCGTTCGG