| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047831 |
| Chromosome: | chromosome 1 |
| Location: | 6930620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049750 | (1 of 1) PTHR12714:SF11 - C-TERMINAL S-ISOPRENYLCYSTEINE CARBOXYL O-METHYLTRANSFERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCAGCTCGGCCGCTTGGCCACAAAATTACAAGCAGCAGGCGACAAG |
| Internal bar code: | TCTTGTTTGTGTTTGTAAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1848 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCGTTTAACTGGAGCCA |
| Suggested primer 2: | AACACGAATTTCTCCGGGCT |