| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047832 |
| Chromosome: | chromosome 2 |
| Location: | 5713172 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116350 | (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTGTGGAATGTCTTGCTGGTGATGTGGTCCATGTAGGCATCCAGGT |
| Internal bar code: | GCGCTCCGTCAATCCTTCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGAGTAACAGTGGCAGT |
| Suggested primer 2: | TGCTTCTTCGATTCGTGCCT |