| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047835 |
| Chromosome: | chromosome 2 |
| Location: | 8469448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142100 | intron | ||
| lncRNA_TCONS_00137062 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTCGAATGGGCTCTGGCAGCAATCGGGAGGCAACAGTCAGGCAACC |
| Internal bar code: | AATTGTCTCCTACCCGCTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 56.7568 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTTCGCTTGGATGACA |
| Suggested primer 2: | GTACACACACACGCACACAC |