Insertion junction: CLIP2.047856_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047856
Chromosome:chromosome 12
Location:2241774
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508650 (1 of 2) PF02010 - REJ domain (REJ) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCACCATCGCCGTCAACATGACGCTGGCGGGCGCCAGCAGCATGAAC
Internal bar code:CCGTCTGGTAAGGTTTCAACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:6562
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGACATCAGGTTGGCGAG
Suggested primer 2:ACCATCACAGTCACCGTGTC