| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047857 |
| Chromosome: | chromosome 2 |
| Location: | 5738372 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116650 | (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAGGTGAGGGGGGCTGACCCCGCGCGCGTCCTCGGGTCACGATGGGA |
| Internal bar code: | AAGCAGTGGAAGTGAGAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGTCATGGGGCATTCA |
| Suggested primer 2: | CCAGCTTCGCCTGTGTTCTA |