Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.047868 |
Chromosome: | chromosome 1 |
Location: | 5887541 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGTCGTGGCCTGCAGACGCCTGCAGGCAGCACAGCTGCAATCCTGC |
Internal bar code: | AAGCGAGTTCGGTCGTCAACTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1266 |
LEAP-Seq percent confirming: | 92.1569 |
LEAP-Seq n confirming: | 47 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCAACCTTTCGCTTGCTGC |
Suggested primer 2: | GCCCCTGTCCTAGCATCATC |