| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047868 |
| Chromosome: | chromosome 14 |
| Location: | 2768395 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626400 | CYP33,CYP743C1 | (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily | CDS |
| Cre14.g626433 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCTCGCCACACGCAGCGAGCTCGAAGGAACTCGTGGTAAATACAGGT |
| Internal bar code: | CTACATAATTTGGGACCAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2112 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGAGTAGGTGCCCTAGG |
| Suggested primer 2: | GGGTTCGGATTTCGGTCTGT |