| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047873 |
| Chromosome: | chromosome 12 |
| Location: | 2502244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507200 | (1 of 2) IPR000104//IPR003613 - Antifreeze protein, type I // U box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTCCGCTCAGGGACCTCAACACCAGCCACGTCATCCTGCAGCTTCA |
| Internal bar code: | GGGCTTCCGTTGGATCGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACCCTCGTCACACCAC |
| Suggested primer 2: | ATGTGGTGTCGTACATCGGG |