| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047888 |
| Chromosome: | chromosome 7 |
| Location: | 4006579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340050 | (1 of 1) K14537 - nuclear GTP-binding protein (NUG2, GNL2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCAAGGAAGGAAAAAGCCGCAACAGCTCTCAAGCCCGGCGAAGGCGA |
| Internal bar code: | TGTATGGTGGTGCAACTATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4152 |
| LEAP-Seq percent confirming: | 94.5205 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCTGGTGAGTCTTTCA |
| Suggested primer 2: | AACGTGTTGATGACCGACGA |