Insertion junction: CLIP2.047888_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.047888
Chromosome:chromosome 7
Location:4006588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g340050 (1 of 1) K14537 - nuclear GTP-binding protein (NUG2, GNL2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTCCGTTGCTACTGTCGCCTGACCGCATGCATGCATTCACGCACATG
Internal bar code:ACTTATTGATATGGCCTGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3018
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTGTTGATGACCGACGA
Suggested primer 2:GCGACCTGGTGAGTCTTTCA