| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047892 |
| Chromosome: | chromosome 12 |
| Location: | 3046125 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501450 | SDIR1 | RING finger-type E3 ligase, SDIR1 ortholog; (1 of 1) PTHR14155//PTHR14155:SF163 - RING FINGER DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGCCGGGTTACGCGCTTGCCCTTTCTGACTCTGAACTCGACAGCTA |
| Internal bar code: | CCTCCGGCGTCATATCGAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4110 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 162 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAATGGGTGCGCTGAAGC |
| Suggested primer 2: | ATCAGCAGCCTCTGTGTTCC |