| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047914 |
| Chromosome: | chromosome 14 |
| Location: | 2003326 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620951 | (1 of 1) PTHR21496//PTHR21496:SF8 - FERREDOXIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATGCACCGCACGGACTTCCATGTAATGCACCGCACGGACTTCCATG |
| Internal bar code: | GAGTAATAATGGGGTGTAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCTTAAGAGTCAGAGCC |
| Suggested primer 2: | ACCGTGTCTGCAAGGAGAAG |