Insertion junction: CLIP2.047933_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.047933
Chromosome:chromosome 11
Location:400880
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467584 (1 of 3) 2.7.11.17//2.7.11.25 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase // Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAAGTGGACGCCGAACGACCTGAGCGAGAGGGGGAAAGGGCGCGCGG
Internal bar code:AGCTAAGGTGAATCATACGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1857
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCTGGCTGGATGCGTCTT
Suggested primer 2:TGAGGGATAAGGGGGACAGG