| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047940 |
| Chromosome: | chromosome 10 |
| Location: | 4254842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449700 | (1 of 7) PF00582 - Universal stress protein family (Usp) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCGATCATAGCCTGCAGAGCAGCAATTTTCAGTACCTCAAGGTGCT |
| Internal bar code: | AAGTTTACTGGCCGCGTGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 73.8095 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCGCACAGGAGCTTGA |
| Suggested primer 2: | CATATTGCAGGTTGAGCCGC |