Insertion junction: CLIP2.047940_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047940
Chromosome:chromosome 16
Location:6122954
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677250 TEH5 (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase; Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAACCGACAGCGAGGGGGACGGTGACCTGTCTAGTGACCGCATCCCAT
Internal bar code:CTGGTTAGATCTATTGGCAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTATGCGCTTCTACCCAT
Suggested primer 2:CTGGCTCCCGAACTTTCTGT