| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047943 |
| Chromosome: | chromosome 12 |
| Location: | 7537385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556400 | (1 of 1) K08066 - nuclear transcription factor Y, gamma (NFYC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGCGCCTCAGCTTGGTGGTGGTTCGGCGGGATTCCACCAGTGTACC |
| Internal bar code: | CATAAAGCGTAGCCGAGTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2809 |
| LEAP-Seq percent confirming: | 84.9057 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTGGCACGGTGATACAA |
| Suggested primer 2: | ACTGTGTGCCGTAGTTGGAG |